FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46274071
Sequences flagged as poor quality0
Sequence length51-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGA500650.024.92397394-95
TCGTGTA677300.024.56051894-95
TGTAGGG541700.024.50912594-95
CGTCGTG788500.024.32240594-95
AGCGTCG932800.024.11589694-95
GTCGTGT731700.024.09565494-95
GCGTCGT841200.024.01195794-95
CGCCGTA28300.023.84952494-95
GTGTAGG636500.023.10885494-95
AGAGCGT1110950.022.82703494-95
TAGGGAA536300.022.62257294-95
AGTGTAG222300.021.84564494-95
GTCGCCG46050.021.80632694-95
GCGTCGG27150.021.22171494-95
AAGAGCG1240550.021.18541194-95
GAGTGTA281200.020.66529894-95
GTAGATC190000.020.23091594-95
GAGCGTC1247750.019.98790794-95
CGTGTAG806200.019.7658294-95
ATCTCGG113900.019.1502594-95
Produced by FastQC (version 0.11.5)