FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51516047
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG2186440.4244192105811224No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT617690.11990244515461368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT444700.026.7071461
TCACGCT464000.025.8628188
AACGCTA477100.024.80248623
CCGGCTT482500.024.7012042
ACGCTAC479050.024.68781924
CGCTACC486600.024.26315125
TTCACGC498750.024.1465517
ACGCTCA507000.023.92669310
AAAACGC504200.023.4984621
GAAAACG510700.023.14204420
CGTGGTT512900.022.87575541
CGGCTTC547650.022.7487163
AAACGCT522250.022.72987622
CACGCTC529100.022.7104429
GTTTTCG516550.022.48283445
TCGTGGT527700.022.21744740
CCTCGTG573350.020.7367738
CGCTCAG597350.020.63541611
TACCTCT589750.020.53469328
CTACCTC586000.020.33203127
Produced by FastQC (version 0.11.5)