FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51516047
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA587150.11397419526385633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC122300.015.4668125
TCACCCG126800.015.441263
AGGGATC174350.014.8596142
CACCCGG142800.014.0524764
ATAGCTC125300.013.8971339
GTTCTAT242100.013.8158691
ACGGACA130600.013.76457918
CGGACAG133800.013.41860519
CGGCCCG141550.013.3911298
AACTAAC186650.013.26112137
CACGGAC140250.013.21813317
TAGCTCT133700.013.19219140
CGGACAC144450.012.94042613
ACTAACC190100.012.92394738
ACACGGA148250.012.55037216
CCGGACA154550.012.47000512
TGATAGC146550.012.43697337
CAGGGAT227450.012.1129431
TTGATAG153400.011.89684436
GACACGG158900.011.69489815
Produced by FastQC (version 0.11.5)