FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50384863
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1885550.37422945855782125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT323550.027.70523545
AGACCGC356600.025.240294
ACCGCGT358100.025.1768176
GACCGCG362850.024.8055325
CCGCGTT368450.024.4390567
CAGACCG373000.024.2571853
CGCGTTC373200.024.1461098
AGAACGA372550.024.11489744
TCAGACC405900.022.5736892
ATACGGA403200.022.232533
AATACGG403700.022.19910232
TACGGAG409550.021.85907734
AAGAACG416800.021.58090443
GCGTTCT419650.021.537769
CAATACG423650.021.18859531
ACGGAGA439100.020.39848735
CCCAATA456750.019.8012129
CTCAGAC505300.019.6964741
TTCGGCG88300.019.55342944
CGGAGAG463400.019.31010436
Produced by FastQC (version 0.11.5)