FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50384863
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG602470.11957361082831564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG191450.014.78885145
GGCATAG217450.012.99693735
GGGAGGT323300.012.7917483
ATAGGGG224250.012.73286538
GGAGGTC321400.012.3417354
ACTCTCC323900.012.06336511
GTCACTC325350.012.0102368
GAGCTTA240400.011.88579644
CATAGGG246000.011.65242237
TGGCATA247950.011.56155134
GGTCACT336950.011.4893627
GCATAGG264450.010.83983936
ACCCGGC82000.010.4537645
TCAGACG97750.010.31391052
AGGTCAC398200.010.2192126
ATAGCTC75150.010.11978539
CGTCGAT23800.010.01999912
TAGGGGA381350.09.98298139
GAGGTCA411550.09.9114415
TGGGAGG316700.09.77052
Produced by FastQC (version 0.11.5)