FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53386060
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT610770.11440627010122117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG38900.024.5779676
TCGACGA45150.021.4248287
CGACGAG44600.020.4784878
GACCGCG170800.018.7629035
CGGTATA67050.018.62143120
ACCGCGT174700.018.4269476
AGACCGC179450.018.0716764
GAACGAT184850.017.72280345
GTTCGAC53750.017.5433885
CCGCGTT183300.017.525587
CGCGTTC190000.016.9312528
CAGACCG194300.016.910563
ACGCTAC142500.015.66227124
AACGCTA141950.015.62712623
CGCTACC147450.014.999207525
AATACGG223200.014.47552932
TACGGAG222000.014.42080534
ATACGGA224050.014.40989433
GTCCGTT35700.014.3769051
ATGACGG85950.014.26452316
Produced by FastQC (version 0.11.5)