FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52136465
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG533670.1023602194740284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT100100.020.1616636
GACCGCG105650.019.1243975
AGACCGC107850.018.6728254
CCGCGTT110300.018.2972137
CGCGTTC120300.016.9258698
CAGACCG125650.016.3487683
TTCGACG20300.016.2925596
CCCGTAT32200.015.960291
GTCCGTT37550.015.9073461
AACGCTA179750.015.70942723
ACGCTAC180300.015.6868424
TCACGCT197900.015.1776138
CGCTACC187200.015.01258325
TCGGCGC59300.014.60742345
GCCGGCT202600.014.5633741
GTCCGAT29650.014.1400211
CCGGCTT237050.014.113912
ACGTTCG16250.013.9841496
ACGCTCA216550.013.88084310
CTACACG67600.013.81239545
Produced by FastQC (version 0.11.5)