FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48997658
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG752520.15358285083748288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA190800.026.44802723
ACGCTAC192750.026.16990924
CGCTACC195550.025.71468225
AAAACGC213750.023.69080421
TCACGCT218950.023.6406568
GAAAACG220950.022.97963320
TTCACGC238050.021.9706347
AAACGCT231550.021.94895222
ACGCTCA255600.020.9813410
GTTTTCG256450.019.94213945
CACGCTC265850.019.571629
GCCGGCT213100.019.0996571
CGTGGTT271300.018.88107741
TCGTGGT272450.018.76009840
CCGGCTT230700.018.691672
CTACCTC291000.017.58985327
TACCTCT294000.017.45630328
CGGCTTC270800.017.1538353
GTCCGTT40150.017.1011331
AGAAAAC302300.016.99660519
Produced by FastQC (version 0.11.5)