FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48997658
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG710980.14510489460537074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG201500.016.47588745
GGCATAG229950.014.52825535
GTTGTCG24550.014.38688626
GGGAGGT330350.014.2629013
ATAGGGG239000.014.07284438
GAGCTTA245800.013.60443744
GGAGGTC343950.013.5554294
TGGCATA253200.013.37190334
ACTCTCC347350.013.28237811
GTCACTC347400.013.09874258
GGTCACT349700.012.9479847
CATAGGG265000.012.72588937
AGGTCAC391750.011.9715176
GCATAGG281000.011.96100936
GAGGTCA402400.011.7605375
TGGGAGG309650.011.4586812
TAGGGGA397450.011.34961439
CACTCTC415000.011.11719110
TCACTCT424900.011.0220799
TGTCGTT33050.010.89099628
Produced by FastQC (version 0.11.5)