FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51018056
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT817940.16032363130417981No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG553930.10857528558124599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT182900.022.7335436
AGACCGC185350.022.5302034
GACCGCG194500.021.4124155
CCGCGTT194800.021.3563397
CGCGTTC198900.020.9274258
CAGACCG203900.020.7011873
GAACGAT197600.020.50744645
TCAGACC241350.018.0111772
TTCGACG17250.017.9998476
AGAACGA233450.017.5413244
GCGTTCT240950.017.36869
ATACGGA239200.017.0536133
TACGGAG240450.016.91821734
AACGCTA196550.016.53000523
ACGCTAC198450.016.34906824
AATACGG251950.016.21736732
CGCTACC204350.015.77794425
CAATACG267100.015.33120631
ACGGAGA267550.015.31390535
AAGAACG273300.014.99167143
Produced by FastQC (version 0.11.5)