FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51018056
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG30050.015.57193726
GGCATAG162800.013.76391135
AGCTTAG168900.013.426208545
ATAGGGG173650.013.20188438
ACCCGGC69750.012.5202045
GGAGGTC261900.012.4784584
GGGAGGT286450.012.3601513
CATAGGG188400.012.00112937
GAGCTTA192600.011.8908844
TGTCGTT40500.011.66527128
TGGCATA199850.011.38046734
GCATAGG200350.011.352736
TAGCGTA10600.011.24881937
TAGGGGA295950.010.60470939
GTCGTTC46150.010.4320429
AGGGATC132200.010.2670862
ACGGACA84850.010.26158318
TCACCCG90200.010.2310733
TATAGCT53750.010.1735297
GCTAAGT48850.010.13341714
Produced by FastQC (version 0.11.5)