FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45668877
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT5352941.1721199100209974No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA2384920.5222199792650912No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC1270880.27828142128390854No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1144170.2505360488719703No Hit
GCCAGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG947040.20737098483941263No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG809730.1773045568867393No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG790640.17312446723837768No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA787000.1723274255243894No Hit
CGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAAT777890.17033263156438028No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG767440.1680444211492216No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTG636130.13929179821960588No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTC500560.10960637372361927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC936250.028.5738374
CAGACCG938550.028.5517643
TCAGACC953600.028.2710462
GACCGCG984600.027.1364045
ACCGCGT1025050.026.0940956
CTCAGAC1095850.025.7089921
TCGGCGC258800.024.44692645
AACGCTA433900.023.65065423
ACGCTAC434850.023.57311424
GTTTTCG374100.023.53999145
TTCGGCG272400.023.4163544
CGTGGTT438550.023.23593941
CGCTACC443150.023.08590725
AAAACGC453750.022.79948621
TCGTGGT451900.022.50967440
GAAAACG461500.022.42148820
AAACGCT462900.022.30993322
TCACGCT498450.020.980578
CCGCGTT1319750.020.26027
AGAAAAC524100.019.84643419
Produced by FastQC (version 0.11.5)