FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54356738
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT985220.1812507586455979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC182750.025.4255074
ACCGCGT189050.024.6352356
GACCGCG194450.023.963865
CAGACCG195200.023.7118283
CCGCGTT197000.023.6182297
CGCGTTC211600.022.0098828
GAACGAT213050.021.65586745
AATACGG224550.020.61071432
ATACGGA231050.020.09879133
AGAACGA233400.019.90270444
TACGGAG235400.019.68915234
TCAGACC247650.019.1259752
CAATACG249200.018.71619231
GCGTTCT252350.018.5527829
AAGAACG259150.017.95116243
ACGGAGA264850.017.4743335
CTCAGAC312250.017.1353241
CCCAATA280100.016.7803129
ATGCGCG34900.016.7668444
TTCGGCG92700.016.50944544
Produced by FastQC (version 0.11.5)