FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51193544
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG551990.10782414282550941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG40050.016.91168826
AGCTTAG195850.015.25361445
GGCATAG200650.015.05244135
ATAGGGG209550.014.4120238
GGTCACT277250.014.2544697
GTCACTC282050.013.9975638
ATATAGC54500.013.9051296
GGAGGTC301400.013.5348114
GGGAGGT317650.013.3656863
AGGTCAC307600.013.3565356
TGGCATA231600.013.26466234
GAGCTTA227300.013.26115844
GAGGTCA325300.012.8646665
ACTCTCC308000.012.76974611
CATAGGG239200.012.69245537
GCTAAGT54750.012.62200214
TATAGCT62800.012.5001917
GCATAGG247300.012.2857736
TGTCGTT62450.011.78476228
TCAGACG84200.011.231642
Produced by FastQC (version 0.11.5)