FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50241229
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT978510.1947623534448172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT204550.023.60654445
AGACCGC221050.022.9755864
GACCGCG224850.022.5772915
ACCGCGT225300.022.5621476
CAGACCG233500.021.904673
CCGCGTT234800.021.6013877
CGCGTTC240300.021.0883358
AGAACGA244950.019.73171444
AATACGG258550.018.69461632
TACGGAG259100.018.64071834
ATACGGA260000.018.60064333
TCAGACC280950.018.5494142
GCGTTCT279300.018.2242019
AAGAACG271650.017.84310743
CAATACG280250.017.28436931
CTCAGAC330450.017.0387951
ACGGAGA299550.016.2094935
CCCAATA313900.015.62412229
CGGAGAG310350.015.56776436
CCAATAC334150.014.85919130
Produced by FastQC (version 0.11.5)