FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50241229
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG113250.011.60875145
GGCATAG118900.010.95935435
ATAGGGG121250.010.92849438
CATAGGG128450.010.36943437
GCATAGG133450.09.99927636
GTTGTCG21850.09.57825726
GTCACTC176550.09.5518028
GGGAGGT213500.09.3137013
GAGCTTA142800.09.31257244
ACCCGGC74600.09.2548655
TGGCATA148900.09.08382934
GGTCACT186800.08.9671737
TTACGCG16250.08.86369735
ATAGCTC74550.08.29878339
GGAGGTC227350.08.1013884
ACGGACA88800.08.08390918
ACTCTCC220000.08.06521111
TAATCGG28750.07.97871264
TAGCGTA6806.002665E-117.940901837
GTAACGA26800.07.72496918
Produced by FastQC (version 0.11.5)