FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51534840
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT645680.1252899979897095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC145050.025.2799974
ACCGCGT148800.024.5978876
GACCGCG149950.024.4388715
CAGACCG160100.023.1855013
CCGCGTT159850.022.8834297
CGCGTTC160450.022.82598
TTCGACG23200.022.4964086
GAACGAT170800.020.16532345
ATACGGA182700.019.8503433
AATACGG184900.019.6749932
TACGGAG183050.019.59117334
TCAGACC202650.019.0394632
GCGTTCT204900.018.0059099
AGAACGA196950.017.55664844
CAATACG210750.017.37898631
TCGACGA30650.017.3218677
CTCAGAC263250.017.042071
ACGGAGA216350.016.72117435
CGGTATA41750.016.32673320
AAGAACG218700.015.85176443
Produced by FastQC (version 0.11.5)