FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51534840
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG575720.11171471571465053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG202050.015.64387335
AGCTTAG202400.015.40060245
GGTCACT293550.014.7950317
GTCACTC293300.014.745828
ATAGGGG223950.014.36378338
GGGAGGT338700.014.1293973
AGGTCAC322750.013.8253876
GAGCTTA229350.013.78046644
CATAGGG230500.013.74119237
ACTCTCC320450.013.71350611
GAGGTCA333150.013.6345145
GGAGGTC329500.013.6015914
TGGCATA237400.013.44807534
GCATAGG244000.013.08280536
GTTGTCG25550.012.41680426
TCACTCT379150.011.7750639
TAGGGGA366900.011.73456939
ACCCGGC89450.011.4244115
CACTCTC384250.011.32005410
AGGGGAG401600.011.01170840
Produced by FastQC (version 0.11.5)