FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50555840
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG882350.17452978726097715No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT559890.11074684942431973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG36500.024.7794156
ACCGCGT124350.022.6525156
GACCGCG125700.022.5885735
AGACCGC128050.022.1388844
GTTCGAC40950.021.8122945
TCGACGA44300.020.7719367
CCGCGTT136750.020.5490887
CGACGAG44950.019.7207748
CGGTATA60400.019.66773820
CAGACCG148150.019.4389613
TCACGCT263900.019.4124838
AACGCTA255000.019.18232523
CCGGCTT282400.019.1210882
ACGCTAC255700.019.11221524
CGCGTTC147550.019.1059788
GCCGGCT255700.018.817281
CGCTACC262200.018.51828225
TTCACGC281800.018.3789947
ACGCTCA287200.018.07259810
AAAACGC277350.017.77344521
Produced by FastQC (version 0.11.5)