FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50555840
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG684630.13542055675466969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT338300.015.4702083
GGAGGTC337950.015.1853244
AGCTTAG250500.014.67518645
GTCACTC355550.014.24581058
GTTGTCG38900.014.22706726
ATAGGGG256900.014.21317638
GGCATAG259550.014.09425835
GAGGTCA374600.014.0059555
GGTCACT359550.013.9192197
AGGTCAC384100.013.5515036
ACTCTCC374400.013.49845611
ATATAGC50000.012.8721826
TGGCATA293150.012.66310434
GAGCTTA295050.012.44405944
TCACTCT418950.012.1918129
CACTCTC418750.012.05275610
TGTCGTT48550.011.72353528
TGGGAGG342850.011.5429652
CATAGGG323750.011.54253837
GCATAGG336350.011.05663336
Produced by FastQC (version 0.11.5)