FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49115289
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG613630.12493665668952901No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA585090.11912583879940114No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG519550.1057817251161853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCGT222650.013.49260544
AGCTTAG109650.012.89339845
TCAGACG124200.012.2637282
GGCATAG115950.012.18465935
GCGTCGA34500.011.08950411
ACCCGGC71900.010.922945
CATAGGG133350.010.91530237
ATAGGGG138050.010.78812738
GAGCTTA133100.010.73576344
ATAGCTC66700.010.69230139
AGGGATC149850.010.5098542
GTCACTC184350.010.4377138
GCATAGG139250.010.37202236
TGCGTCG38200.010.36888610
CGTCGAT35900.010.28089312
GCCCGTG299200.010.23278145
TGGCATA139750.010.2061534
ACTCTCC199950.09.79219811
CAGACGT155300.09.7357743
CTGCCCG325000.09.61044243
Produced by FastQC (version 0.11.5)