FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48167352
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT657700.13654476999275358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT130000.025.7702756
GACCGCG135150.024.7383355
AGACCGC136250.024.7037454
CCGCGTT138750.024.112697
CAGACCG149650.022.6721293
CGCGTTC148800.022.423638
GAACGAT172350.019.69906645
TCAGACC190650.018.6815032
AATACGG192400.017.57606332
ATACGGA188600.017.54844733
GCGTTCT192550.017.4688939
TACGGAG187850.017.41489634
TTCGACG18750.017.3994056
AGAACGA199500.017.04077144
CTCAGAC265150.015.85786151
CAATACG215200.015.81846631
TTCGGCG69350.015.34554844
ACGGAGA216850.015.18970635
AAGAACG225900.015.13892643
AACGCTA75500.014.84055223
Produced by FastQC (version 0.11.5)