FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40572652
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGG52650.011.7101794
ATAGGGG95000.011.4853438
ATCGGTT56000.011.4517376
ATAATCG55050.011.4040263
GGCATAG97100.010.79674735
GTAACGA57700.010.49072518
GGTAACG57350.010.35889417
AGCTTAG105600.010.20424245
GTTGTCG17900.09.8041926
CATAGGG109750.09.6957737
TCGGTTT67850.09.6509017
GGGAGGT145450.09.591563
GCATAGG111300.09.52032936
AATCGGT67400.09.4813655
GAGCTTA115950.09.467998544
TCAGACG79550.09.3904332
CGGTTTC67450.09.1409928
TGGCATA116000.09.11518734
CGTGGCG96650.08.8929771
GGAGGTC152800.08.7914864
Produced by FastQC (version 0.11.5)