FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53564536
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1720550.32121066072522314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT348600.024.9583021
AACGCTA399050.024.66763723
ACGCTAC399800.024.62113224
CGCTACC406450.024.19073525
TCACGCT424750.023.6392778
AAAACGC424750.023.21694821
GAAAACG428200.023.09802420
TTCACGC436850.023.082367
CCGGCTT403050.022.9673522
AAACGCT431000.022.93286322
ACCGCGT86750.022.9003246
ACGCTCA455350.022.5250110
CACGCTC459800.021.9302489
CGTGGTT453750.021.75641841
GTTTTCG450450.021.7258545
GACCGCG94300.021.0448885
TCGTGGT469000.020.95686340
AGACCGC94650.020.8958684
CGGCTTC469000.020.4567453
CTACCTC490500.020.2427227
Produced by FastQC (version 0.11.5)