FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53564536
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG933640.1743018925805686No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN649370.12123133111803674No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA627510.11715027271028727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT410200.016.9774073
GGAGGTC418450.016.513214
AGCTTAG295950.015.68719645
ATAGGGG299600.015.629601538
GGCATAG296450.015.59949535
GGTCACT450800.015.0685987
GTCACTC458200.015.0125258
ACTCTCC464850.014.84652511
TGGCATA327750.014.20534234
GAGCTTA329700.014.14788344
AGGTCAC511900.013.537836
GAGGTCA522350.013.4260195
TGGGAGG369000.013.0814162
CATAGGG360550.012.92554737
GTTGTCG25000.012.87075226
CACTCTC540750.012.79126210
TCACTCT546000.012.7634319
CACCGAA372150.012.47514645
GCATAGG385250.012.12080236
AGCATAG180350.012.1162345
Produced by FastQC (version 0.11.5)