FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51200911
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1119940.21873438931584635No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT612940.11971271370542606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG26200.023.3585266
AGACCGC128450.022.981574
ACCGCGT131850.022.440036
GACCGCG136100.021.7392945
CCGCGTT147250.020.077887
CGCGTTC151950.019.4272338
CAGACCG154350.019.4167943
TCACGCT334550.019.1740578
TCGACGA32700.018.3025557
ACGCTAC345800.017.9971524
AACGCTA345900.017.96592923
TTCACGC360350.017.8699327
GCCGGCT340750.017.795651
ACGCTCA367550.017.75856410
CCGGCTT374100.017.6221872
CGGTATA40350.017.50905220
CGCTACC353500.017.46510525
CACGCTC374050.017.1431949
AAAACGC371100.016.81262421
CGACGAG33400.016.7064068
Produced by FastQC (version 0.11.5)