FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51200911
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA675100.13185312269150837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG167000.015.00790745
GGCATAG172350.014.553925535
ATAGGGG183500.013.69422738
CATAGGG190400.013.28066337
TGGCATA199000.012.76307534
GGGAGGT287600.012.7286693
GTCACTC261000.012.6835698
GAGCTTA198850.012.671885544
GTTGTCG24300.012.59063226
GCATAGG203700.012.35829736
ACTCTCC273750.012.32947311
GGTCACT268400.012.2422517
ACCCGGC82450.012.0942785
GGAGGTC287250.012.0218124
AGGGATC154200.011.72442
TCAGACG92850.011.4693462
TGCGTCG27600.011.33230210
ACGGACA88500.011.16004218
CGTCGAT26700.011.03914912
GCGTCGA28150.010.63115911
Produced by FastQC (version 0.11.5)