FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50105626
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA131200.023.28945223
ACGCTAC134850.022.69253724
TTCGACG29400.022.5738956
CGCTACC142650.021.42060525
AAAACGC153150.020.12658521
TCACGCT164550.019.5647188
GAAAACG164300.018.85657720
AAACGCT168700.018.2581622
TCGACGA38700.017.4979577
ACGCTCA195450.017.46145410
CGGTATA48800.017.01269120
ACCGCGT56650.016.7191436
TTCACGC197000.016.638927
CGACGAG41500.016.3715828
GCCGGTT101050.016.1542971
GTTTTCG190600.016.14800545
GCGGACT104100.016.1459852
GTCCGTT33900.015.7628411
ACGTTCG18900.015.7124516
CACGCTC205050.015.6894639
Produced by FastQC (version 0.11.5)