FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50105626
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG733830.1464566074875504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG215900.016.0929145
GTTGTCG33450.015.33939626
GGCATAG231550.014.84462835
GTCACTC324250.014.072578
ATAGGGG247700.014.00563738
GAGCTTA250050.013.9578944
GGTCACT322700.013.8888777
GGGAGGT356700.013.4283783
TGGCATA267100.013.06296934
CATAGGG269400.012.81926537
TGTCGTT40550.012.76534428
ACTCTCC360400.012.72932811
GGAGGTC367400.012.7287984
AGGTCAC373350.012.3842856
GAGGTCA381950.012.3358435
GCATAGG282550.012.2317536
ATATAGC48350.012.093196
GCTAAGT45450.011.87827314
TCACTCT400100.011.5492319
CACTCTC405200.011.3089310
Produced by FastQC (version 0.11.5)