FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50095291
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG924150.18447841734266002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG25600.019.9477256
GCCGGCT272400.018.9716511
CCGGCTT294400.018.815842
TCACGCT276000.018.6897078
TTCACGC289400.018.0652967
AACGCTA281200.017.86626423
AGACCGC96000.017.858594
ACGCTAC281000.017.82309524
ACCGCGT96000.017.785996
CGCTACC288850.017.26954825
GACCGCG102150.016.6931515
ACGCTCA316900.016.67509310
CGGCTTC345750.016.5614573
AAAACGC307500.016.4116721
CAGACCG108500.016.2577063
GAAAACG318550.015.88429320
CACGCTC331900.015.7249119
AAACGCT325300.015.57547322
GTTTTCG325350.015.41192945
TCGACGA33600.015.2652197
Produced by FastQC (version 0.11.5)