FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51847042
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT846660.16329957647342735No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG545300.10517475616063111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC166450.025.8286444
ACCGCGT176250.024.3924986
GACCGCG176450.024.3265975
CAGACCG181600.023.8597073
CCGCGTT187100.022.9779667
CGCGTTC190350.022.5738268
GAACGAT204100.020.604245
TCAGACC226950.019.707192
GCGTTCT221400.019.4384699
TACGGAG220750.018.9485634
AATACGG224450.018.74627532
ATACGGA227250.018.57388133
CTCAGAC274350.018.2907491
AGAACGA232150.018.15707244
CAATACG234850.018.06946831
ACGGAGA249300.016.84164235
AAGAACG251650.016.79482343
CGGAGAG260350.016.03173636
GCCGGCT190400.015.8439781
CCCAATA276150.015.66814529
Produced by FastQC (version 0.11.5)