FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51925124
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT613620.1181740076345316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC149200.021.8508474
GACCGCG149400.021.535465
ACCGCGT153400.021.0912466
CAGACCG162350.020.4274373
CCGCGTT162850.019.812087
TTCGACG23200.019.6868726
CGCGTTC168050.019.2659748
GAACGAT184400.017.64314845
TCAGACC209350.016.4862212
AATACGG205150.015.86972832
TACGGAG206550.015.5660934
ATACGGA209700.015.5575833
CCCGTAT32900.015.2711561
GCGTTCT215300.015.1527939
CCGTACG55950.015.1201624
CTCAGAC266400.014.9524031
CGTACGC56450.014.9463815
CAATACG223800.014.71815831
AGAACGA223500.014.60691744
CGGTATA41800.014.58685620
Produced by FastQC (version 0.11.5)