FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51960518
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG31800.09.5535226
ACCCGGC67650.09.2418915
CGTAACG14450.09.18717442
TAATCGG69100.08.8201324
ATCGGTT73700.08.7249716
GTAACGA65950.08.7009518
ATAGGGG83350.08.66393438
TCACCCG81100.08.4597643
CACCCGG76650.08.2741014
CGGCCCG80500.08.1840088
ATAATCG79250.08.089563
CTAGAAT105650.07.921381
GGCATAG86000.07.90310135
ACGGACA80300.07.790593618
CATAGGG82900.07.73495737
GGTAACG75950.07.70368617
GCATAGG83700.07.662179536
TTAACGC45300.07.651458332
AATCGGT83850.07.5636245
TCGGTTT90700.07.5361247
Produced by FastQC (version 0.11.5)