FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50218796
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG874760.17418975954740132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG48050.027.1100226
TCGACGA56200.023.4588097
CGACGAG55700.023.386658
GTTCGAC59550.022.1393265
CGGTATA75400.021.30508220
TCACGCT304400.019.6673078
CCGGCTT282600.019.259622
TTCACGC315400.019.0955127
GCCGGCT254400.018.9290921
GACCGCG93400.018.403275
AACGCTA317700.018.40002823
ACGCTAC319800.018.3004224
ACCGCGT94050.018.2519996
AGACCGC95100.018.0047934
CGCTACC326150.017.94413625
ACGCTCA338650.017.90409510
AGTTCGA77250.017.679674
ATGACGG89300.017.45928416
AAAACGC343900.017.10171521
CGGCTTC333350.016.9889583
Produced by FastQC (version 0.11.5)