FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55909830
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1067640.1909574756353221No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG978440.1750032149981497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG31400.025.142176
AACGCTA220500.024.49400323
ACGCTAC222950.024.19560824
CGCTACC231200.023.2446825
AAAACGC246250.021.94028721
GAACGAT245950.021.20588345
GAAAACG256450.021.17258320
TCACGCT264950.021.17188
GACCGCG250700.020.915525
AGACCGC252250.020.8763714
ACCGCGT252800.020.7480266
AAACGCT265400.020.40046522
CCGCGTT262200.019.995627
CGCGTTC266000.019.709978
CAGACCG270650.019.681993
TCGACGA41650.019.2787347
CCGGCTT303100.018.995462
TTCACGC301850.018.7103027
ACGCTCA307650.018.67193410
GTTTTCG297400.018.08271645
Produced by FastQC (version 0.11.5)