FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48404672
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG136650.014.65409645
GGCATAG149400.013.46515535
ATAGGGG158550.012.858397538
CATAGGG165050.012.1611537
GAGCTTA172750.011.89204344
GCATAGG172500.011.75330736
GTCACTC214600.011.5853928
GGGAGGT240000.011.3636433
GGTCACT219450.011.2475587
TGGCATA183400.011.09158234
GGAGGTC243000.010.6953214
ACTCTCC238150.010.6756411
AGGTCAC241800.010.6646676
GAGGTCA250850.010.5040525
CACCGAA203050.010.32741345
GCACCGA211700.09.76783844
TCAGACG95100.09.72650052
TAATCGG35800.09.176734
TAGGGGA268650.09.16344839
CGTGGCG149250.09.140271
Produced by FastQC (version 0.11.5)