FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57332695
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG717330.12511709069319No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT666000.1161640840361682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT169450.019.6909316
AGACCGC171050.019.4541974
TCGGCGC92600.018.15044645
CCGCGTT185600.018.0138917
CAGACCG187450.017.8845213
GACCGCG190100.017.516525
CGCGTTC192550.017.3637338
GTCCGTT34800.016.425011
GAACGAT202050.016.26930845
AACGCTA243550.016.12989423
ATGCGCG30750.016.02422544
ACGCTAC245950.015.99994124
TTCGGCG108100.015.88096644
TCAGACC220750.015.5230022
TCACGCT263400.015.4095028
CGCTACC255700.015.37225425
ACGTTCG20200.015.3707266
TGCGCGT32450.015.25406645
GCCGGCT269350.015.1304771
AAAACGC264500.014.93737221
Produced by FastQC (version 0.11.5)