FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57332695
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG595040.10378720204937165No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA587330.10244241963507908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG208400.016.36720345
GGCATAG226500.014.95061535
GTTGTCG26850.014.91584726
GAGCTTA249850.013.84144
TGGCATA248800.013.71854834
ACCCGGC101450.013.6394485
GGAGGTC376200.013.4806124
GGGAGGT406000.013.3502633
ATAGGGG262550.013.21485338
TCACCCG110550.012.8425013
CATAGGG273350.012.49518537
GCATAGG282800.012.12538636
CCGGACA114850.011.89168812
ACGGACA119550.011.763411518
CGGACAC115050.011.71502113
ATAGCTC112400.011.67062539
ACACGGA115550.011.62538316
CGTCGAT34550.011.331490512
TGCGTCG34300.011.28251210
ACTCTCC408750.011.24557511
Produced by FastQC (version 0.11.5)