FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57233865
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1631910.28513014104499146No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG702670.12277171915613247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT408400.023.42375445
ACCGCGT416100.022.9440576
GACCGCG412850.022.3820115
AACGCTA220250.022.07575223
CCGCGTT434050.022.0184697
AGACCGC407300.021.9530224
ACGCTAC223350.021.77485824
CGCGTTC440700.021.6861828
CAGACCG417450.021.0154443
CGCTACC234450.020.7630525
AGAACGA481450.019.89628844
TCAGACC448800.019.7382952
AAAACGC249500.019.67544221
TTCGACG29550.019.6452316
GCGTTCT490450.019.559829
GAAAACG262200.018.81673620
AATACGG505050.018.70109732
AAACGCT263550.018.66923122
ATACGGA510100.018.5115133
TACGGAG509500.018.46208834
Produced by FastQC (version 0.11.5)