FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50878907
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA600560.1180371268588769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG32750.016.41747926
AGCTTAG162850.015.33505845
ATAGGGG168450.015.14471438
GGCATAG169100.014.80696135
GTCACTC240200.013.950438
CATAGGG185100.013.62442637
TGTCGTT40300.013.62072928
GAGCTTA187250.013.54090244
GGGAGGT284450.012.9801643
GCATAGG194550.012.974136
TGGCATA196650.012.86974434
ACTCTCC265250.012.83477911
ATATAGC50600.012.6764676
AGGTCAC286850.012.2554736
GAGGTCA290600.012.2459785
GGAGGTC290000.012.2334464
GGTCACT278450.012.1154857
GTCGTTC44600.011.90399629
TAGGGGA290450.011.479156539
TATAGCT56450.011.4021957
Produced by FastQC (version 0.11.5)