FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53361813
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1017710.19071878236221096No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT989590.18544909634161044No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG758550.14215221660478441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG31350.024.1144246
AGACCGC244750.021.7963834
CGGTATA50150.021.35534920
CAGACCG259500.020.6355063
GACCGCG265400.020.1597925
AACGCTA279800.019.84575823
ACGCTAC283000.019.5816424
ACCGCGT276750.019.3330046
TCGACGA39550.019.2282817
CGCTACC293100.018.92231425
AAAACGC300900.018.53636621
GAAAACG312750.017.84124220
AAACGCT316350.017.70220422
TCAGACC322000.017.2380752
GTTTTCG324450.016.8652445
CGACGAG44150.016.8171928
CGTGGTT338700.016.28189341
TCGTGGT343350.016.00228940
CTCAGAC388200.015.8783451
CCGCGTT337050.015.8606997
Produced by FastQC (version 0.11.5)