FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46659790
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3506080.7514135833015965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG146300.015.7287845
GGCATAG155950.014.73886735
ATAGGGG160300.014.35228238
CATAGGG170750.013.57872637
GTTGTCG31300.013.48783326
GAGCTTA172200.013.45324744
TGGCATA177450.013.11659934
GCATAGG183300.012.71046936
ACTCTCC260600.011.88657111
GGGAGGT289300.011.8002253
GGAGGTC273900.011.6631954
GTCACTC273600.011.4640728
TGTCGTT36450.011.45684428
GGTCACT270450.011.30011757
TAGGGGA272000.011.13531139
ATATAGC45600.010.5331316
TATAGCT49500.010.38021857
ACCCGGC74100.010.2805155
GCTAAGT44100.010.08610314
ATAGCTC75150.09.8975339
Produced by FastQC (version 0.11.5)