FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47030211
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG688010.14629107234921826No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA667180.14186200440393515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG27300.013.26035726
GGGAGGT256100.012.8831693
TCAGACG124550.012.6134582
AGCTTAG189150.012.47067945
CGTCGAT39250.011.96921212
GGAGGTC272400.011.8152284
GGCATAG200650.011.76153335
ATAGGGG204300.011.68786838
TGCGTCG40450.011.61054810
ATATAGC35050.011.4748256
GCGTCGA40600.011.40373111
TAGCGTA6700.011.08246137
CGTGGCG215750.010.833861
TGGCATA224000.010.77237634
TATCCTA100200.010.7656445
GAGCTTA221900.010.75201644
CGGCTCG54100.010.4691043
CAGACGT148900.010.2339013
ACTCTCC302100.010.22213911
ATCCTAG110400.09.9725926
Produced by FastQC (version 0.11.5)