FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45276055
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1299180.2869463781683276No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT613760.13555951374297076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG27250.025.8432526
GCCGGCT338150.023.8833771
TCGACGA32350.022.4645427
TCACGCT414900.021.089388
AACGCTA402800.020.77891223
ACGCTAC401300.020.76126924
CCGGCTT432750.020.406662
CGCTACC406150.020.3360825
AAAACGC423950.019.72106621
TTCACGC449800.019.5731057
GAAAACG429550.019.47444220
CGGCTTC478400.019.174273
AAACGCT446500.018.79562222
CACGCTC458650.018.7784569
ACGCTCA463200.018.6037110
CGTGGTT441000.018.35148441
CGACGAG38100.018.306538
GTTTTCG444900.018.13498545
TCGTGGT447550.018.07285140
CGGTATA44350.017.29936220
Produced by FastQC (version 0.11.5)