FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45276055
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG488630.10792238855615843No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA464820.10266353815499163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG42250.018.6908126
TGTCGTT49350.015.54576328
GGGAGGT300800.015.5010043
GGAGGTC287900.015.4213134
GTCACTC285500.015.1463798
TCAGACG90700.015.0107782
GAGGTCA299150.014.8996725
ATATAGC56150.014.8660256
GCTAAGT54700.014.4372914
GGTCACT302800.014.3628777
AGGTCAC310850.014.3602336
TATAGCT63250.014.0508317
ACCCGGC83250.013.7026635
AGCTTAG246000.013.61036345
ACTCTCC319750.013.59446111
TGGGAGG265600.013.4971732
GGCATAG248900.013.288856535
TCGTTCC58850.013.26603830
CGTTCCC59650.012.89975831
GTCGTTC61550.012.64723929
Produced by FastQC (version 0.11.5)