FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53484477
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT597660.11174457216810776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT127600.025.635186
GACCGCG127300.025.5718865
AGACCGC129650.025.3513054
CCGCGTT138400.023.6347457
CAGACCG145750.022.7978883
CGCGTTC149600.021.8803378
TCAGACC186950.018.327962
GCGTTCT191700.017.2159359
GAACGAT188150.017.10813745
AATACGG184450.016.83442132
TACGGAG194150.016.50323534
CTCAGAC254950.016.230381
ATACGGA200400.016.21232433
AACGCTA177350.015.95779523
ACGCTAC177500.015.881577524
CGCTACC182050.015.41088725
AGAACGA209550.015.37904844
CAATACG209650.015.068531
TTCGACG15600.014.709516
ACGGAGA220500.014.67379235
Produced by FastQC (version 0.11.5)