FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65704308
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG929430.1414564780135878No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT779520.11864062246877327No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC703450.10706299501700862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC128050.023.8266074
ACCGCGT142800.021.5386496
GACCGCG148550.020.7201675
CAGACCG148100.020.6773593
CCGCGTT157450.019.5201247
CGCGTTC160850.019.1906818
AACGCTA255000.018.87237723
ACGCTAC257500.018.724124
TGCGCGT75350.018.1246445
CGCTACC268500.017.94024725
AATACGG170500.017.86701432
GAACGAT160850.017.806245
GTTAGTT221300.017.66994744
TCACGCT283300.017.5589898
ATACGGA176050.017.4443333
ATGCGCG77350.017.36524644
TCGGCGC212350.017.32325445
TTCGGCG217850.017.17519244
AAAACGC285650.017.0679921
ACGTTCG35050.016.9470486
Produced by FastQC (version 0.11.5)