FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65704308
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG758940.11550840775919899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC166750.016.7862685
TCACCCG180700.016.0617583
AGCTTAG247800.014.97661945
GGCATAG255850.014.73346935
CACCCGG206800.013.8283264
CGGACAC207350.013.65164513
CCGGACA207250.013.62568212
ACTCTCC409300.013.60120611
ACGGACA204850.013.42513118
ATAGCTC197700.013.3958139
TGGCATA282800.013.37710534
CGGACAG207400.013.325809519
GTCACTC422950.013.3160328
TCAGACG154950.013.2219732
GGTCACT418900.013.2194337
GAGCTTA285100.013.0722544
GGGAGGT487900.013.0668323
TAGCGTA22350.012.97481937
ACACGGA218250.012.67266616
TAGCTCT210050.012.5865340
Produced by FastQC (version 0.11.5)