FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40329840
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG643240.1594948058311166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT25500.019.5219021
ACGTTCG15250.018.737196
CTCGGTT76200.018.6823771
TCACGCT218850.018.6800928
GCCGGCT204600.018.6719721
AACGCTA194600.018.34869823
ACGCTAC196250.018.20589324
CGCTACC201250.017.69767225
TTCGACG15600.017.595676
TTCACGC236900.017.437267
CCGGCTT248800.017.3176522
CACGCTC238000.017.2054529
AAAACGC209400.017.08405921
GCGGACT62250.016.9513422
GAAAACG236200.016.93645520
ACGCTCA245900.016.90899510
AAACGCT229900.015.58025822
CGGCTTC296200.015.5566293
CCCGTAT26300.015.3308881
CTCCGAT40450.015.2581561
Produced by FastQC (version 0.11.5)