FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40329840
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG122000.015.23074345
GGCATAG131350.013.87263935
GTTGTCG22450.013.3283926
ATAGGGG143250.013.03424738
ACCCGGC67700.012.4985155
GAGCTTA150050.012.33859844
TGGCATA150850.012.24342834
GGGAGGT210800.012.1592093
GCGTCGT23700.012.05936111
CATAGGG156450.011.87700737
GGAGGTC205200.011.5370544
GCATAGG163900.011.33715736
CGGACAC76200.011.16356913
TAGCGTA8000.010.9667337
ACGGACA75950.010.9623618
GGTCACT212500.010.8339067
GTCACTC221250.010.6190488
CCGGACA82200.010.56779712
ACTCTCC225950.010.53761311
TCGTGTA28200.010.53381514
Produced by FastQC (version 0.11.5)